Clinical Specimen Submission Guidance

Submit all specimens to 50 Orms St, Providence RI 02915. Open Monday to Friday 8:30 AM to 4:30 PM

Ebola Hemorrhagic Fever PCR

Lab Special Pathogens & Biothreats Laboratory 401-222-5586
Organism Ebola virus
Disease Ebola Hemorrhagic Fever
Test Description CDC Ebola Virus Real-Time RT-PCR Assays performed at the RI State Health Laboratories (or other CDC designated lab)
Special Instructions

Pre-approval for testing is mandatory. Physician must consult with Health Department Center for Acute Infectious Disease Epidemiology at (401) 222-2577 immediately if Ebola virus infection is suspected. See CDC guidance for Patient Under Investigation (PUI) definition:

CDC Specimen Collection Guidance is located at the following link:


Collect in Lavender top (EDTA) plastic tube

Minimum of 4.0 ml whole blood

Note: if collected in 3 ml tube, 2 tubes must be drawn (1.0 ml minimum acceptable for infants)

Rejected: Specimen collected in glass tube

CLIA regulations require two patient identifiers on the specimen container and the test requisition

Form Required

Rhode Island State Health Laboratories (RISHL) Test Requisition Form

Test Request

Special Pathogens Primary Specimen

Write in "Ebola testing" on comment line of requisition form

Transport After collection, specimens are to be transported to your hospital's laboratory in accordance with CDC Collection Guidance (see above link) where they will be appropriately packaged in compliance with federal regulations as Category A Infectious Substances and transported to the RI State Health Laboratories.
Normal Value Specimen negative for Ebola virus RNA
Reporting Requirement Providers must report patient information on Ebola Hemorrhagic Fever immediately using the RIDOH reportable disease form.
Turnaround Time Approximately 4 hrs. from specimen receipt at State Laboratories