0.5 mL of serum and/or 1.0 mL of CSF is required for serology testing. Specimen must be kept cold at 2° - 8° C or frozen at <-20° C.
Acute 3 to 10 days after onset of symptoms
Convalescent 2-3 weeks after acute sample
Virus isolation and/or nucleic amplification testing, acceptable specimens are fresh frozen tissue, serum, or cerebrospinal fluid. Tissue specimens should be approximately 1 x 3 cm, frozen as soon as possible at -70° C
CLIA regulations require two patient identifiers on the specimen container and the test requisition.