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Mumps PCR

Clinical Specimen Submission Guidance

Submit all specimens to 150 Richmond Street, Suite 100, Providence, RI 02903. Open Monday to Friday 8:30 AM to 4:30 PM.   Learn how and where to drop off your lab samples at our Laboratory Central Services webpage.

Lab

Molecular Diagnostics Laboratory 401-222-5538

Analyte Name

Mumps

Disease

Mumps

Test Description

This test is performed using a laboratory developed TaqMan real-time reverse transcription-PCR assay for the detection of the nucleoprotein gene present in the respective Measles and Mumps virus genomes. The performance characteristics of this assay were validated by the Rhode Island State Health Laboratories. It has not been approved nor cleared by the U.S. Food and Drug Administration. Results are for epidemiological and investigational purposes only and may not be used as a substitute for diagnostic procedures nor to guide patient management decisions.

Special Instructions

PREAUTHORIZATION by RIDOH Center for Acute Infectious Disease Epidemiology (401) 222-2577 is required prior to transporting specimens.

Specimen

Preferred specimen: Buccal swab

Obtain by massaging the parotid glad area for 30 seconds prior to swabbing area around Stenson's duct (http://www.cdc.gov/mumps/lab/detection-mumps.html)

Use a PCR grade (synthetic) tipped swab to collect specimen and immediately place the swab into 2 mL viral transport medium (VTM) or universal transport medium (UTM).

Ream swab around rim of tube, break swab off into tube and tighten cap securely.

Store refrigerated at 2-8°C. If transport is delayed beyond 24 hours, specimens must be frozen at -20°C or -70°C.

 

Specimen Identification

CLIA regulations require specimens be labeled with at least two patient identifiers. Examples of identifiers are first and last name, date of birth, chart/medical record number. The specimen container must be labeled to match the test requisition or the electronic order.

Form Required

Rhode Island State Health Laboratories (RISHL) test requisition form https://health.ri.gov/forms/LabRequisitionForm.pdf

Test Request

Mumps PCR

Transport

Transport within 24 hours after collection.

Refrigerated: Transport and deliver to the laboratory within 24 hours of collection at 2-8°C in a cooler able to maintain specimen temperature. A plug-in electric cooler is recommended, however, a cooler packed with excess frozen gel packs is acceptable as long as the transport temperature is maintained at 2-8°C. 

Frozen: If specimen previously frozen, it must be transported on frozen gel packs in insulated shipper.

Specimens must be transported and packaged in accordance with current federal shipping regulations

Specimen Rejection Criteria

The following rejection criteria will be used to ensure accurate specimen information and specimen condition for testing:

  • Specimen received outside acceptable transport range
  • Demographics different / Unable to read identifiers on specimen
  • Two identifiers required on specimen
  • Expired / Incorrect collection kit
  • Insufficient quantity
  • Urine cup received
  • No specimen received
  • Specimen leaked in transit
  • Test not evaluated in adolescents <14
  • Specimen too old
Reporting Requirement

Providers must report patient information on Mumps (PCR) within 4 days using the RIDOH reportable disease form.

Turnaround Time

1 business day