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Dengue virus

Clinical Specimen Submission Guidance

Submit all specimens to 50 Orms St, Providence RI 02904. Open Monday to Friday 8:30 AM to 4:30 PM

Lab

STI & Arbovirus Laboratory 401-222-5591

Analyte Name

Dengue Virus

Disease

Dengue (Dengue Fever) 

Test Description

Testing performed at CDC:

Acute serum collected within 7 days from onset of symptoms is suitable for testing by Real-time Polymerase Chain Reaction (RT-PCR) and Enzyme Linked Immunosorbent Assay (ELISA).

Convalescent serum > 7 days from onset of symptoms is tested by Enzyme Linked Immunosorbent Assay (ELISA) for IgM antibodies.

Special Instructions

PREAUTHORIZATION by RIDOH Center for Acute Infectious Disease Epidemiology (401) 222-2577 is required prior to transporting specimens.

All specimens must be submitted to the CDC through State Health Departments and not submitted directly to CDC.

Specimen

Acute serum: 0-7 days post onset date

Convalescent serum: > 7 days post onset

Serum: collect blood in red top tube or serum separator tube, spin, pour off serum and submit >=2 mL in a sterile plastic screw-capped tube.

Store serum at 2° - 8°C.

If specimen cannot be transported to RISHL within 24 hours, then the serum should be frozen at or below -20°C.

Specimen Identification

CLIA regulations require specimens be labeled with at least two patient identifiers. Examples of identifiers are first and last name, date of birth, chart/medical record number. The specimen container must be labeled to match the test requisition or the electronic order.

Form Required

Rhode Island State Health Laboratories (RISHL) test requisition form https://health.ri.gov/forms/LabRequisitionForm.pdf

CDC 50.34 Form

CDC Dengue Case Investigation Form

Test Request

Dengue Serology

Indicate on the test requisition whether the serum is acute or convalescent

Transport

Transport specimens as soon as possible.

Contact the RISHL STI & Arbovirus Lab (401) 222-5591 prior to transport.

Refrigerated: Transport and deliver to the laboratory within 24 hours of collection at 2-8°C in a cooler able to maintain specimen temperature. A plug-in electric cooler is recommended, however, a cooler packed with excess frozen gel packs is acceptable as long as the transport temperature is maintained at 2-8°C. 

Frozen: If specimen previously frozen, it must be transported on frozen gel packs in insulated shipper.

Specimens must be transported and packaged in accordance with current federal shipping regulations.

Normal Value

Negative for Dengue virus by PCR

Negative for IgM antibodies to Dengue virus

Reporting Requirement

Providers must report patient information on Dengue (1,2,3,4) within 4 days using the RIDOH reportable disease form.

Turnaround Time

Performed at CDC: 10 days

Kit

Containers specifically labeled for transport of specimens and isolates to the RISHL STI & Arbovirus Laboratory are available in all Serology Laboratories and Laboratory Sendout Departments.