Clinical Specimen Submission Guidance

Submit all specimens to 50 Orms St, Providence RI 02915. Open Monday to Friday 8:30 AM to 4:30 PM

Zika virus infection

Lab Bioterrorism Response & Special Pathogens Lab 401-222-5586
Organism Zika virus
Disease Zika
Test Description Trioplex PCR and Zika IgM MAC-ELISA. Testing performed is based on patient clinical history, specimen source and collection date. Serology may be performed on specimens collected >=4 days after symptom onset as well as on asymptomatic patients who are approved for testing. PCR is performed on serum and CSF specimens collected <=7 days after symptom onset as well as on urine collected < 14 days from symptom onset. Due to known cross reactivity among the flaviviruses, testing for Dengue and Chikungunya will also be performed on serum specimens from symptomatic patients. Specimens with positive or equivocal IgM results will be forwarded to CDC for PRNT.
Special Instructions Pre-approval for testing is mandatory. Medical provider must consult with the Department of Health Center for Acute Infectious Disease Epidemiology (401) 222-2577 if Zika virus infection is suspected.
Specimen

Serum: collect blood in a serum separator tube, spin, pour off serum and submit >=1.0 ml in a sterile screw capped plastic tube.

Also accepted only when accompanied by a matching serum:

Urine: >=1.0 ml in a sterile screw capped plastic tube

CSF: >= 0.5 ml in a sterile screw capped plastic tube

Store at 2- 8C and transport to RISHL on an ice pack as soon as possible. Specimens must be received within 4 days of collection.

CLIA regulations require two patient identifiers on the specimen container and the test requisition

Form Required

Rhode Island State Health Laboratories (RISHL) test requisition form

Test Request

Special Pathogens Primary Specimen

Write in "Zika testing" on comment line of requisition form

Transport Package with an ice pack and in accordance with federal regulations for Biological Substance Category B.
Normal Value Negative for Zika virus
Turnaround Time

PCR and Serolgy = 5 days

PRNT (performed at CDC)