Submit all specimens to 50 Orms St, Providence RI 02915. Open Monday to Friday 8:30 AM to 4:30 PM
|Lab||Molecular Diagnostics Laboratory 401-222-5538|
Real-Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR) for the detection of Influenza RNA
Specimens are only accepted from designated RI Sentinal Sites that are approved by the RIDOH Center for Acute Infectious Disease Epidemiology (401) 222-2577.
All other laboratories must obtain PREAUTHORIZATION by the RIDOH Center for Acute Infectious Disease Epidemiology (401) 222-2577 prior to transporting specimens.
Refer to the Influenza Specimen Collection Kit Instructions for specimen collection.
Collect nasopharyngeal specimens with a flexible wire shaft nasopharyngeal (NP) swab.
Collect throat specimens with a plastic shaft swab with a polyester, Dacron or rayon tip
The NP swab and the throat swab can be placed in the same tube of VTM
After specimen is obtained, inoculate the tube of Viral Transport Media (VTM) by inserting the swab into the tube making certain that the liquid in the tube covers the swab tip. The shaft should be clipped equal with the top of the tube.
Store refrigerate at 2-8°C for up to 72 hours. If unable to transport within 72 hours, the specimen must be frozen at -70°C.
CLIA regulations require two patient identifiers on the specimen container and the test requisition
Rhode Island State Health Laboratories (RISHL) test requisition form.
Write "FLU" in comment field on requisition form.
Specimens must be transported on wet ice or equivalent cooling material and packaged in accordance with current federal shipping regulations.
If specimen previously frozen it must be transported on dry ice.
Containers specifically labeled for transport of specimens to the RISHL Molecular Diagnostics Laboratory are available in all microbiology labs and send out departments.
|Normal Value||PCR Negative for Influenza A and B|
|Turnaround Time||PCR result within 48 to 96 hours|