Arbovirus Testing

Mission

To aid in the diagnosis of insect-borne virus (arbovirus) infections, such as by Zika virus, Dengue virus, Chikungunya virus, and West Nile virus (WNV). In addition, to monitor for the presence of mosquito-borne arboviruses WNV and Eastern Equine Encephalitis virus (EEE) in mosquitoes trapped and collected from throughout the state by the Department of Environmental Management (DEM).

What we do

  • Test trapped mosquitoes, pooled by species, during mosquito season (June October) for the presence of arboviruses WNV and EEE using advanced molecular methods.
  • Share test results with the RIDOH Center for Acute Infectious Diseases and Epidemiology (CAIDE) and DEM to assist them with surveillance for the presence and spread of arboviruses within the state. report
  • Provide clinical diagnostic testing for Zika virus, Dengue virus, and Chikungunya virus using advanced molecular-based test methods.
  • Provide clinical diagnostic testing for Zika virus, Dengue virus, Chikungunya virus, and West Nile Virus (WNV) using serological (antibodies)-based test methods.

Accomplishments

  • Cell culture methods for arboviral detection were replaced by advanced nucleic acid extraction and rRT-PCR molecular amplification methods for the screening of trapped mosquito pools for arboviruses.
  • During the 2017 mosquito season, the SHL tested 1533 pools of mosquitoes and identified 10 pools infected with arboviruses (0.65% positivity).
  • The combined number of Zika tests (rRT-PCR and Serology) peaked at 1108 specimens tested.